![]() There is a type of bread enjoyed daily by millions across the Indian subcontinent and around the world. So if these are such competent stackers, and they are, why aren’t we using them? To understand that, an analogy is in order. At the current time, there are two commercially available, affordable, stacking products that offer all or most of the required functionality and an intuitive graphic user interface Helicon Focus and Zerene Stacker. So the technical challenge should be self evident - how do we blend these one thousand partially focused images into one fully focused one? We need a sophisticated focus stacking software program to do the heavy lifting. Let that constraint sink in for a moment - to build a composite image of a subject one millimeter deep, made up of images that are all in sharp focus, would require that the camera or subject move roughly one micron between capturing each of a thousand individual frames. At a magnification of 20X, for example, our depth of field is only around 2 microns, or 2 thousandths of a millimeter. The primary difficulty in capturing high resolution images of very small subjects using photographic equipment is a result of the very shallow depth of field at which we work.
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